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Hero.Coli is a biology-themed adventure game. It is designed to help discover and learn about synthetic biology. In the game, the player explores a rich environment by controlling a bacterium, modifies its abilities, and even crafts new skills using BioBricks™ they collected. No prior knowledge in biology required. Hero.Coli is a free and online computer game that contributes to research in game-based learning. In order to so, game data are anonymously gathered through an open-source, open data, home-developed analytics service. These data are then combined with survey data, and analyzed using open-source Python scripts.